This Standard sets out a method for determining the most probable number (MPN) of spores of sulfite-reducing anaerobes (clostridia) including C.perfringens in water, using a multiple tube dilution technique, with a confirmation procedure for identifying C.perfringens.
Refer to AS 4276.1 for a description of the general procedure for estimating the MPN of microorganisms.
MPN procedures may be used for the examination of all water samples and are particularly applicable for samples containing sufficient suspended material to make filtration procedures impractical.
NOTE: A flow diagram of the procedure is shown in Appendix A.
